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KMID : 1123920150290020195
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Korean Journal of Oriental Physiology and Pathology
2015 Volume.29 No. 2 p.195 ~ p.201
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Anti-cancer Effects of Scutellaria barbata in AGS Human Gastric Adenocarcinoma Cells
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Sim Ji-Hwan
Lee Su-Jin
Kim Hee-Jin
Park Hyun-Soo
Kim Byung-Joo
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Abstract
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The aim of the study is to investigate the anti-cancer effects of Scutellaria barbata in AGS human gastric adenocarcinoma cells. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and caspase 3 or 9 activity assay were carried out to examine cell death with Scutellaria barbata . To elucidate the inhibitory effects of Scutellaria barbata , cell cycle (sub-G1) analysis and mitochondrial membrane potential were performed in AGS cells after 24 h incubation with Scutellaria barbata . Scutellaria barbata induced apoptosis in AGS cells by using the MTT assay, the sub-G1 analysis and mitochondrial membrane potential assay. The stronger inhibition effects of AGS cell growth was observed by application of Scutellaria barbata combined with several anti-cancer drugs (paclitaxel, 5-fluorouracil, cisplatin, ectoposide, doxorubicin and docetaxel) in comparison to the application of Scutellaria barbata or anti-cancer drugs. Our findings provide insight into unraveling the effects of Scutellaria barbata in human gastric cancer cells and developing therapeutic agents against gastric cancer.
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KEYWORD
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Cutellaria barbata, Human gastric adenocarcinoma cancer cell, AGS, Anti-cancer drugs
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